We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Purification, Characterization and Protective Effects of Indigenous Yeast Derived ß-glucans against Salmonellosis in Broilers.
- Authors
Anwar, Muhammad Irfan; Tahir, Muhammad Ali; Awais, Mian Muhammad; Raza, Ahmad; Akhtar, Masood; Muhammad, Faqir; Hameed, Muhammad Raza; Ijaz, Nabeel
- Abstract
Indigenous Saccharomyces (S.) cerevisiae was cultured under submerged fermentation conditions and ß-glucans were extracted by enzymatic method. The extracted ß-glucans were characterized by FT-IR and their spectra presented peaks at a wavelength of 1021 cm-1. Safety and mutagenicity testing of ß-glucans showed them non-mutagenic and non-toxic up to a concentration of 200 mg/mL. For immunotherapeutic effects against Salmonella (Sal.) typhimurium infection, broiler chicks (n=120) were randomly divided into 4 equal groups viz. A (negative control), B (positive control challenged with Sal. typhimurium; 1.0× 1010 cfu), C (ß-glucans at the dose rate of 50 mg/kg feed + Sal. typhimurium challenge), and D (ß-glucans at the dose rate of 100 mg/kg feed + Sal. typhimurium challenge). Results revealed protective effects of ß-glucans against Sal. typhimurium infection as depicted by mild clinical signs along with minimal/no gross and histopathological lesions. Immunological responses were also higher (P<0.05) in ß-glucans administered birds. ß-glucans also modulated intestinal morphology with improved villi-height and crypt-depth ratio. In conclusion, ß-glucans isolated from indigenous S. cerevisiae conferred protective effects against salmonellosis and therefore can be used successfully in broilers.
- Subjects
SALMONELLA diseases; MUTAGENICITY testing; SALMONELLA; POULTRY growth; YEAST; SYMPTOMS; SALMONELLA typhimurium
- Publication
Pakistan Veterinary Journal, 2023, Vol 43, Issue 2, p297
- ISSN
0253-8318
- Publication type
Article
- DOI
10.29261/pakvetj/2023.013