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- Title
Peridinin–chlorophyll–protein reconstituted with chlorophyll mixtures: Preparation, bulk and single molecule spectroscopy
- Authors
Brotosudarmo, T.H.P.; Hofmann, E.; Hiller, R.G.; Wörmke, S.; Mackowski, S.; Zumbusch, A.; Bräuchle, C.; Scheer, H.
- Abstract
Abstract: Reconstitution of the 16kDa N-terminal domain of the peridinin–chlorophyll–protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that contain Chl a in one pigment cluster, and Chl b in the other. Binding of Chl b is stronger than that of the native pigment, Chl a. Energy transfer from Chl b to Chl a is efficient, but there are only weak interactions between the two pigments. Individual homo- and hetero-chlorophyllous complexes were investigated by single molecule spectroscopy using excitation into the peridinin absorption band and scanning of the Chl fluorescence, the latter show frequently well resolved emissions of the two pigments.
- Subjects
SPECTRUM analysis; PHOTOBIOLOGY; GASES from plants; NUCLEAR reactions
- Publication
FEBS Letters, 2006, Vol 580, Issue 22, p5257
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.08.049