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- Title
Confirming the Genetic Identity of Dendrobium fimbriatum Using an Amplification Refractory Mutation System (ARMS).
- Authors
Shan Lu; Xiaoyu Ding; Yanhong Ma; Li Han; Weichao Zhang; Liang Qian; Zhenyu Feng; Feng Zhang
- Abstract
The quality of herbal medicine is important for ensuring consumers’ safety and efficacy. As a valuable herbal medicine, Dendrobium fimbriatum is adulterated by other Dendrobium species. However, it is extremely hard to identify D. fimbriatum and its adulterants from their morphological and chemical features. In order to establish an effective method, the psbA–t rnH intergenic spacer was used to identify D. fimbriatum and its common adulterant. The divergences existed in that sequence could be utilized for the molecular marker of Dendrobium interspecies. Furthermore, two amplification refractory mutation system (ARMS) diagnostic primers of D. fimbriatum have been designed based on the psbA–t rnH intergenic spacers. PCR amplification of D. fimbriatum with ARMS diagnostic primer LSJB2 was conducted successfully. Thirty dried herb samples labeled as D. fimbriatum were identified by LSJB2. It demonstrated that psbA–t rnH region and ARMS were simple and practical ways in herb identification.
- Subjects
CONSUMER protection; DENDROBIUM; GENETIC mutation; HERBS; NUCLEOTIDE sequence
- Publication
Plant Molecular Biology Reporter, 2010, Vol 28, Issue 4, p712
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1007/s11105-010-0190-4