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- Title
Bioinformatics characterization of patatin-related phospholipase A (pPLA) gene family in agriculturally important crops viz Vigna radiata, Vigna angularis, and Glycine max.
- Authors
Parveen, Reshma; Vaish, Swati; Gupta, Divya; Basantani, Mahesh K.
- Abstract
Patatin-related phospholipases A (pPLA) are involved in diverse plant metabolic processes, ranging from normal organ development to managing biotic and abiotic stresses. In the current study, the pPLA gene family was characterized in Vigna radiata, Vigna angularis, and Glycine max. A total of 13, 13, and 22 pPLA genes were identified in V. radiata, V. angularis, and G. max, respectively. The protein lengths varied from 91 to 1667 amino acid residues, the pI value ranged between 4.71 to 9, and molecular weight was between 10.12 to 185.64 (kDa), in the three crops. The conserved patatin domain was towards the N- terminus in the identified pPLA proteins. Subcellular localization showed that the pPLA proteins were present in the cytoplasm, mitochondria, extracellular space, plasma membrane and chloroplast. Gene organization of the pPLAs was in conformation with the canonical gene architecture of the pPLA gene family. Multiple Expectation Maximization for Motif Elicitation (MEME) analysis showed the presence of significantly conserved motifs in the pPLA proteins. Phylogeny analysis led to the clustering of the pPLAs in the three different groups (I, II and III) in accordance with their classification. The results of the current study will be of significant importance in the molecular and functional characterization of this gene family.
- Subjects
SOYBEAN; GENE families; VIGNA; AMINO acid residues; MUNG bean; MOLECULAR weights
- Publication
Biologia, 2022, Vol 77, Issue 5, p1429
- ISSN
0006-3088
- Publication type
Article
- DOI
10.1007/s11756-022-01026-6