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- Title
Cold shock protein RBM3 attenuates atrophy and induces hypertrophy in skeletal muscle.
- Authors
Van Pelt, Douglas W.; Confides, Amy L.; Judge, Andrew R.; Vanderklish, Peter W.; Dupont-Versteegden, Esther E.
- Abstract
RNA-binding motif protein 3 (RBM3), a stress-inducible RNA-binding protein that increases protein synthesis and confers cell protection in multiple cell types, has been identified as a possible regulator of skeletal muscle mass. Therefore, the primary aim of this study was to examine the impact of elevated RBM3 on skeletal muscle hypertrophy and resistance to atrophy. Plasmid-mediated overexpression of RBM3 in vitro and in vivo was used to assess the role of RBM3 in muscle. C2C12 myotubes overexpressing RBM3 were approximately 1.6 times larger than non-transfected myotubes, suggesting a role for RBM3 in hypertrophy. In addition, elevated RBM3 attenuated atrophy in myotubes exposed to dexamethasone. In agreement with in vitro results, overexpression of RBM3 in soleus muscle of F344/BN rats using electroporation techniques increased the cross sectional area of muscle fibers. Overexpression of RBM3 also attenuated muscle atrophy in rat soleus muscle undergoing disuse atrophy. These findings provide direct evidence for a novel role of RBM3 in inducing hypertrophy as well as attenuating atrophy.
- Subjects
SKELETAL muscle; MYOSIN; HYPERTROPHY; MUSCULAR dystrophy; GENE expression
- Publication
Journal of Muscle Research & Cell Motility, 2018, Vol 39, Issue 1/2, p35
- ISSN
0142-4319
- Publication type
Article
- DOI
10.1007/s10974-018-9496-x