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- Title
Identification and genetic characterization of new bovine viral diarrhea virus genotype 2 strains in pigs isolated in China.
- Authors
Tao, Jie; Wang, Yin; Wang, Juan; Wang, Jian-ye; Zhu, Guo-qiang
- Abstract
Classical swine fever (CSF)-like symptoms in pigs regarded as free from CSF has been reported previously. From sick pigs with CSF-like symptoms, and who had been inoculated with the hog cholera vaccine, samples were collected and subjected to RT-PCR using specific primers. Twelve bovine viral diarrhea virus 2 (BVDV-2) strains were screened and isolated. Homology comparison showed that the E2 genes of the twelve isolates were highly conserved. The genome of the one of the BVDV-2 isolates (named as SH-28) from the sick pigs, which showed a noncytopathic effect in MDBK cell cultures and strong reactivity with monoclonal antibody (MAb) Bz-53 raised against BVDV-2, was sequenced. The genome of SH-28 comprises 12,279 nucleotides and contains a large open reading frame beginning at nucleotide 386 and ending at nucleotide 12,073. Genomic comparison and phylogenetic analyzes showed that SH-28 fall into BVDV-2 subtype and was most similar to XJ-04 (nucleotide and amino acid homologies were 89.9-93.8 % and 91.1-96.9 %, respectively), but was genetically divergent from ZM-95 (pig BVDV-1).
- Subjects
CHINA; CLASSICAL swine fever; VIRUS diseases in swine; PESTIVIRUS diseases; CHOLERA vaccines
- Publication
Virus Genes, 2013, Vol 46, Issue 1, p81
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-012-0837-3