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- Title
Visualization of Plastids in Pollen Grains: Involvement of FtsZ1 in Pollen Plastid Division.
- Authors
Lay Yin Tang; Noriko Nagata; Ryo Matsushima; Yuling Chen; Yasushi Yoshioka; Wataru Sakamoto
- Abstract
Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.
- Subjects
PLASTIDS; PLANT cells &; tissues; POLLEN; CELL division; PLANT genetics; TRANSGENIC plants; FLORAL morphology
- Publication
Plant & Cell Physiology, 2009, Vol 50, Issue 4, p904
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pcp042