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- Title
A novel psbA mutation (Phe274–Val) confers resistance to PSII herbicides in wild radish (Raphanus raphanistrum).
- Authors
Lu, Huan; Yu, Qin; Han, Heping; Owen, Mechelle J; Powles, Stephen B
- Abstract
BACKGROUND: Wild radish (Raphanus raphanistrum) is a globally important weed of crops. Two atrazine‐resistant wild radish populations (R1 and R2), collected from the Western Australia grain belt, were investigated for resistance to photosystem II (PSII) herbicides. RESULTS: Sequencing of the full‐length psbA gene revealed the well‐known Ser264–Gly substitution in population R1, whereas population R2 displayed a novel Phe274–Val substitution. Herbicide dose–response studies confirmed that the population with the Ser264–Gly mutation exhibited high‐level resistance to atrazine, but super‐sensitivity to bromoxynil. Plants possessing the novel Phe274–Val mutation exhibited a modest level of resistance to atrazine, metribuzin and diuron, and were bromoxynil susceptible. Structural modelling of the mutant D1 proteins predicts that the Ser264–Gly mutation endows atrazine resistance by abolishing H‐bonds, but confers bromoxynil super‐sensitivity by enhancing hydrogen bonding. The Phe274–Val substitution provides resistance to atrazine and diuron by indirectly affecting H‐bond formation between the Ser264 residue and the herbicides. CONCLUSION: The results demonstrate that the Phe274–Val mutation is likely responsible for resistance to PSII‐inhibiting triazine and urea herbicides. To our knowledge, this is the first evidence of the psbA Phe274–Val mutation in wild radish conferring resistance to PSII herbicides. © 2018 Society of Chemical Industry This study identifies a novel psbA Phe274–Val mutation conferring photosystem II (PSII) herbicide resistance. Structural modelling predicts that this mutation endows resistance by reducing H‐bonds between herbicides and the D1 protein.
- Subjects
HERBICIDE resistance; RAPHANUS raphanistrum; WEED control; VEGETATION management; BIOLOGICAL weed control
- Publication
Pest Management Science, 2019, Vol 75, Issue 1, p144
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.5079