We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Non-Histone Proteins and the Mediator Complex are Essential for the Transcription of HXT2 and HXT4 Genes in Saccharomyces cerevisiae.
- Authors
Güzelvardar, Sinem; Türkel, Sezai
- Abstract
Transcriptional activation of HXT2 and HXT4 genes require rapid reorganization of the chromatin structure on the promoter regions of these genes. In this study, it was found that the transcription of HXT2and HXT4genes decreased 4- to 7-fold in the yeast strain that does not contain the non-histone proteins 6A and 6B (Nhp6A/B). In addition, results of this research indicated that Nhp6A/B are required for the rapid derepression and activation of HXT2 and HXT4 transcription upon glucose signaling. Furthermore, it was found that Med2p, which provides the connection between the transcriptional activators and the basal transcription factors, is also required for the activation of HXT2 and HXT4 transcription. Transcription of HXT2 and HXT4 decreased 2- to 13-fold in Med2 mutant S. cerevisiae cells. These results indicated that Nhp6A/B and the mediator complex are essential for the regulation of HXT2 and HXT4 gene transcription in S. cerevisiae.
- Subjects
SACCHAROMYCES cerevisiae; GLUCOSE; HISTONES; ENDOMYCETALES; MONOSACCHARIDES; SUCROSE
- Publication
Turkish Journal of Biology, 2006, Vol 30, Issue 3, p163
- ISSN
1300-0152
- Publication type
Article