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- Title
Cloning and Expression of the Isoflavone Synthase Gene (IFS-Tp) from Trifolium pratense.
- Authors
Kim, Bong Gyu; Kim, Song-Young; Song, Hee Su; Lee, Chan; Hur, Hor-Gil; Kim, Su-ll; Ahn, Joong-Hoon
- Abstract
Isoflavones are secondary metabolites found mainly in leguminous plants. ]'heir synthesis from flavanones is catalyzed by isoflavone synthase (IFS). We have cloned a isoflavone synthase gene (IFS-Tp) from Trifolium pratense that encodes a predicted 525 amino acids protein, molecular weight 59 kDa, with strong homology to IFS's from other legumes. IFS-Tp was expressed in all the tissues examined, and addition of glutathione and UV irradiation enhanced its expression. Micro somes from yeast transformed with IFS-Tp were able to convert naringenin to genistein, indicating that IFS-Tp has isoflavone synthase activity.
- Subjects
ISOFLAVONES; METABOLITES; MOLECULAR genetics; RED clover; AMINO acids
- Publication
Molecules & Cells (Springer Nature), 2003, Vol 15, Issue 3, p301
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13741-1