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- Title
Molecular Cloning and Functional Expression of Bovine Brain GABA Transaminase.
- Authors
Seong Gyu Jeon; Jae Hoon Bahn; Joong Sik Jang; Sang Ho Jang; Byung Ryong Lee; Kil Soo Lee; Jinseu Park; Tae-Cheon Kang; Moo Ho Won; Hyong Bai Kim; Oh-Shin Kwon; Sung-Woo Cho; Soo Young Choi
- Abstract
We isolated a cDNA that encodes the bovine brain γ-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the λgt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10- kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial- expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.
- Subjects
AMINOTRANSFERASES; PROTEINS; GABA; GENE expression; CATTLE
- Publication
Molecules & Cells (Springer Nature), 2001, Vol 12, Issue 1, p91
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)17066-x