We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Protective effect of the Baicalin against DNA damage induced by ultraviolet B irradiation to mouse epidermis.
- Authors
Zhou Bing-Rong; Jin Song-Liang; Chen Xiao-E; Lin Xiang-Fei; Cai Bao-Xiang; Gao Jie; Luo Dan
- Abstract
Purpose: To investigate whether topical application of Baicalin affords protecting Balb/C mice epidermis from ultraviolet (UV)B-induced DNA damage and its underlying mechanisms. Methods: A DNA damage model of UVB irradiation-induced mice epidermis was established. The immunohistochemical staining, Southwestern dot-blotting were used for cyclobutane pyrimidine dimers (CPDs) detection; Western blotting was used for p53 detection; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of Bcl-2 and Bax; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to detect apoptotic cells. Results: Topical application of Baicalin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 1, 24 and 48 h after 180 mJ/cm2 of UVB irradiation as compared with untreated mice. UVB-induced apoptosis was less pronounced in Baicalin-treated mice epidermis, which was accompanied by less p53 accumulation and higher Bcl-2/Bax ratio compared with that of untreated mice. Conclusion: Taken together, these results suggest that topical Baicalin application mitigates DNA photo-damage. Baicalin is therefore a promising protective substance against UVB radiation.
- Subjects
DNA damage; MICE; EPIDERMIS; ULTRAVIOLET radiation; BIOCHEMICAL genetics
- Publication
Photodermatology, Photoimmunology & Photomedicine, 2008, Vol 24, Issue 4, p175
- ISSN
0905-4383
- Publication type
Article
- DOI
10.1111/j.1600-0781.2008.00356.x