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- Title
Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions.
- Authors
Cooper, Kendal G.; Chong, Audrey; Starr, Tregei; Finn, Ciaran E.; Steele-Mortimer, Olivia
- Abstract
Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined with a synthetic transcriptional terminator, and plasmid copy number variation. This yielded a series of plasmids that drive uniform production of fluorescent and endogenous proteins, over a wide dynamic range. We describe several examples where this system is used to fine-tune constitutive expression in Salmonella, providing an efficient means to titrate out toxic effects of protein production.
- Subjects
EPISOMES; PROTEIN expression; GENETIC transcription; SALMONELLA; PLASMIDS
- Publication
Frontiers in Cellular & Infection Microbiology, 2017, Vol 7, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2017.00475