We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Radioimmunological detection of anti-transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow-up.
- Authors
BONAMICO, M.; NENNA, R.; LUPARIA, R. P. L.; PERRICONE, C.; MONTUORI, M.; LUCANTONI, F.; CASTRONOVO, A.; MURA, S.; TURCHETTI, A.; STRAPPINI, P.; TIBERTI, C.
- Abstract
Background Serum radioimmunoassay (RIA) tissue transglutaminase autoantibodies (tTG-Abs) proved to be a sensitive test also during coeliac disease (CD) follow-up. We demonstrated that RIA tTG-Abs could be detected in human saliva. Aim To evaluate salivary RIA tTG-Abs in coeliac children on gluten-free diet (GFD). Methods Saliva and serum samples from 109 coeliac children were evaluated at diagnosis (group 1: 71 females, median age 9.4 years) and 58 of them on GFD: 36 after 3–6 months (group 2a), 34 at 9 months or more (group 2b). Two gender- and age-matched control groups: 89 gastroenterological patients (group 3) and 49 healthy subjects (group 4) participated in the study. Saliva and serum tTG-Abs were detected by RIA and compared with serum tTG-Abs ELISA and IgA anti-endomysium antibodies (EMA). Results Salivary RIA tTG-Abs were found in 94.5%, 66.7% and 50.0% of groups 1, 2a and 2b CD patients and in 98.2%, 72.2% and 50.0% of corresponding serum samples, respectively. tTG-Abs decreased with GFD progression and a correlation was found between saliva and serum titres ( r = 0.75, P = 0.0001). During the CD follow-up, salivary and serum RIA sensitivities were comparable, and higher with respect to EMA and ELISA. Conclusions This study demonstrates that it is possible to detect salivary tTG-Abs with high sensitivity not only at CD diagnosis, but also during GFD.
- Subjects
RADIATION immunology; RADIOIMMUNOASSAY; TRANSGLUTAMINASES; BLOOD coagulation factors; AUTOANTIBODIES; SALIVA analysis; CELIAC disease; DIGESTIVE system diseases
- Publication
Alimentary Pharmacology & Therapeutics, 2008, Vol 28, Issue 3, p364
- ISSN
0269-2813
- Publication type
Article
- DOI
10.1111/j.1365-2036.2008.03720.x