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- Title
Inactivation of the CTD phosphatase-like gene OsCPL1 enhances the development of the abscission layer and seed shattering in rice.
- Authors
Hyeonso Ji; Sung-Ryul Kim; Yul-Ho Kim; Hakbum Kim; Moo-Young Eun; Il-Doo Jin; Young-Soon Cha; Doh-Won Yun; Byung-Ohg Ahn; Myung Chul Lee; Gang-Seob Lee; Ung-Han Yoon; Jung-Sook Lee; Yeon-Hee Lee; Seok-Cheol Suh; Wenzhu Jiang; Jung-Il Yang; Ping Jin; McCouch, Susan R.; Gynheung An
- Abstract
Although susceptibility to seed shattering causes severe yield loss during cereal crop harvest, it is an adaptive trait for seed dispersal in wild plants. We previously identified a recessive shattering locus, sh-h, from the rice shattering mutant line Hsh that carries an enhanced abscission layer. Here, we further mapped sh-h to a 34-kb region on chromosome 7 by analyzing 240 F2 plants and five F3 lines from the cross between Hsh and Blue&Gundil. Hsh had a point mutation at the 3′ splice site of the seventh intron within LOC_Os07g10690, causing a 15-bp deletion of its mRNA as a result of altered splicing. Two transferred DNA (T-DNA) insertion mutants and one point mutant exhibited the enhanced shattering phenotype, confirming that LOC_Os07g10690 is indeed the sh-h gene. RNA interference (RNAi) transgenic lines with suppressed expression of this gene exhibited greater shattering. This gene, which encodes a protein containing a conserved carboxy-terminal domain (CTD) phosphatase domain, was named Oryza sativa CTD phosphatase-like 1 ( OsCPL1). Subcellular localization and biochemical analysis revealed that the OsCPL1 protein is a nuclear phosphatase, a common characteristic of metazoan CTD phosphatases involved in cell differentiation. These results demonstrate that OsCPL1 represses differentiation of the abscission layer during panicle development.
- Subjects
PHOSPHATASES; ABSCISSION (Botany); NUCLEIC acids; DNA; CELL differentiation
- Publication
Plant Journal, 2010, Vol 61, Issue 1, p96
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2009.04039.x