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- Title
Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour.
- Authors
Phuong, Nguyen Thi Mai; Cuong, Trinh Tat; Quang, Dang Ngoc
- Abstract
Objective To evaluate the anti-inflammatory activity of methyl ferulate (MF) isolated from the roots of Stemona tuberosa ( S. tuberosa ) Lour (Stemonaceae) in lipopolysaccharide activated macrophage cells. Methods Methanol extracts of a root powder of S. tuberosa were prepared for isolation of a potential anti-inflammatory agent using ultrasound extraction combined with repeated chromatography on silica gel. After the quantitative analyses, anti-inflammatory activity of the isolated compound was evaluated by measurement of cytokine release, NO generation, expression of cyclooxygenase-2 and phosphorylation of mitogen activated protein kinases including p38 and c-Jun NH2-terminal kinase using quantitative kits and Western blotting with specific antibodies. Results The isolation process yielded a potential anti-inflammatory compound with a purity level of 99% determined by high performance liquid chromatography. The compound was identified as MF by using nuclear magnetic resonance. MF strongly inhibited the release of pro-inflammatory cytokines from macrophages, including IL-6, TNFα, IFNγ, yet it did not affect the anti-inflammatory cytokine IL-10. Phosphorylation of p38 and c-Jun NH2-terminal kinase were clearly reduced in MF-treated macrophages stimulated with lipopolysaccharide. cyclooxygenase-2 expression and NO generation by macrophages were also suppressed when the cells were treated with MF. Conclusions The data suggested that MF is a possible inhibitor of the mitogen activated phosphor kinase pathway and could be a potential anti-inflammatory agent isolated for the first time in medicinal plant S. tuberosa .
- Publication
Asian Pacific Journal of Tropical Medicine, 2014, Vol 7, pS327
- ISSN
1995-7645
- Publication type
Article
- DOI
10.1016/S1995-7645(14)60254-6