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- Title
Distribution of Wheat-Infecting Viruses and Genetic Variability of Wheat Streak Mosaic Virus and Barley Stripe Mosaic Virus in Kazakhstan.
- Authors
Kapytina, Anastasiya; Kolchenko, Mariya; Kerimbek, Nazym; Pozharskiy, Alexandr S.; Nizamdinova, Gulnaz; Taskuzhina, Aisha; Adilbayeva, Kamila; Khusnitdinova, Marina; Amidullayeva, Malika; Moisseyev, Ruslan; Kachiyeva, Zulfiya; Gritsenko, Dilyara
- Abstract
Wheat is an essential cereal crop for the economy and food safety of Kazakhstan. In the present work, a screening of wheat and barley from different regions of Kazakhstan was conducted using newly developed specific primers for reverse transcription PCR and loop-mediated isothermal amplification (LAMP) assays. In total, 82 and 19 of 256 samples of wheat and barley tested positive for wheat streak mosaic virus (WSMV) and barley stripe mosaic virus (BSMV), respectively. A phylogenetic analysis using two independent methods revealed that most of the analyzed isolates had a European origin. Molecular data on the distribution and diversity of cereal viruses in Kazakhstan were obtained for the first time and will help lay a foundation for the implementation of genetics and genomics in wheat phyto-epidemiology in the country.
- Subjects
KAZAKHSTAN; MOSAIC viruses; VIRAL variation; GENETIC variation; FOOD safety; WHEAT genetics; BARLEY; BARLEY yellow dwarf viruses; WHEAT
- Publication
Viruses (1999-4915), 2024, Vol 16, Issue 1, p96
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v16010096