We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of Rapid PCR Methods for the Detection and Quantification of Geosmin-Producing Dolichospermum spp.
- Authors
Miao, Hanchen; Zhang, Ji; Shen, Qingyue; Ichise, Satoshi; Asada, Yasuhiro; Tian, Yuan; Utsumi, Motoo; Lei, Zhongfang; Zhang, Zhenya; Takanashi, Hirokazu; Fujimoto, Naoshi; Nishimura, Osamu; Akiba, Michihiro; Shimizu, Kazuya
- Abstract
Geosmin, produced by cyanobacteria and actinomycetes, is a common cause of earthy odor in lakes, rivers, and reservoirs. Drinking water quality standards in Japan requires that geosmin concentration be maintained at < 10 ng/L. The concentration of musty/earthy odor compounds in water bodies tends to exhibit a positive correlation with the cell density of cyanobacteria producing these compounds. In general, it is difficult to accurately distinguish and quantify musty/earthy odor-producing cyanobacteria through microscopic observation. Thus, it is essential to develop simple and rapid monitoring methods to assess the abundance of musty/earthy odor-producers and predict the onset of musty odor events to ensure good water quality. In this study, we developed PCR and qPCR-based assays using whole cells to effectively monitor geosmin-producing cyanobacteria using a gene encoding geosmin synthase (geoA). Geosmin-producing cyanobacteria Dolichospermum smithii NIES-824 and Dolichospermum macrosporum were used as representative strains for the study. The brightness of the specific electrophoresis band related to geoA gene amplified using whole-cell PCR was found to be positively correlated (R2 > 0.9) to the cell density of Dolichospermum spp. In addition, the whole-cell qPCR analysis presented a high correlation (R2 > 0.8) as well, between the copy number of geoA gene and cyanobacterial biomass. These results affirm the potential of using whole-cell PCR and qPCR-based detection methods for the rapid prediction and monitoring of geosmin producers in aquatic environments.
- Publication
Water, Air & Soil Pollution, 2022, Vol 233, Issue 9, p1
- ISSN
0049-6979
- Publication type
Article
- DOI
10.1007/s11270-022-05859-4