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- Title
Investigation of Anti-inflammatory Activity and Metabolite Content of Methanolic Aquilaria Malaccencis Leaves Extract.
- Authors
Ibrahim, Noorhuda; Huq, AKM Moyeenul; Tajuddin, Saiful Nizam
- Abstract
Aquilaria or agarwood leaves has gained a proactive interest among researchers due to its variety of secondary metabolites contents which reported to be effective as alternative treatment in many diseases. Even while agarwood leaf research is moving forward at a rapid pace, there is still a lack of empirical data to support the stated pharmacological effects, notably the anti-inflammatory effects. Therefore, this study aims to investigate the antiinflammatory potential of Aquilaria Malaccencis leaf extract. Leaves were extracted in methanol by maceration. Both in vitro and in vivo tests were performed by BSA denaturation inhibition method and in mice paw edema model respectively. The phytochemical profiling for volatile compounds was conducted by GCMS while the nonvolatile compounds was screened using UPLC-QToF/MS. The in vitro study revealed that the extracts exhibited concentration-dependent inhibition of protein (albumin) denaturation across the concentration range of 31.25-1000 µg/ml examined. The in vivo result revealed that the extract reduced the paw edema significantly (0.406 ±0.054 mL, p<0.05) after 4 hr pre-treatment observation as compared to the disease group (0.542±0.031 mL). The phytochemical analysis of the bioactive extract by GCMS identified 48 compounds were identified and n-hexadecanoic acid (27.06%) was the major compounds present in the extract. Furthermore, the presence of phenolic compounds including xanthones, benzophenone and flavones from UPLC-QToF/MS analysis may inspire further investigation on anti-inflammation mechanism, as well as its toxicity regarding its potential as an alternative therapeutic source for medicinal purposes.
- Subjects
ANTI-inflammatory agents; XANTHONE; METABOLITES; PHENOLS; EXTRACTS; THERAPEUTICS
- Publication
Current Trends in Biotechnology & Pharmacy, 2023, Vol 17, p44
- ISSN
0973-8916
- Publication type
Article