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- Title
A New Screening Approach for Glycolipid-type Biosurfactant Producers Using MALDI-TOF/MS.
- Authors
Shun Sato; Tokuma Fukuoka; Azusa Saika; Tatsuyuki Koshiyama; Tomotake Morita
- Abstract
We applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDITOF/MS) to screen for glycolipid-type biosurfactant (BS) producers. A crude extract of Pseudozyma antarctica, a well-known mannosylerythritol lipid (MEL) producer, was initially subjected to MALDI-TOF/MS. The spectrum of the extract showed the accumulation of diacylated MELs in culture. We then screened 80 environmental samples for BS-producing yeasts, and extracts from broth cultures of the selected five strains were examined using MALDI-TOF/MS. The results showed that all five strains produced MELs, whereas four strains also produced cellobiose lipids (CLs). By D1/D2 region sequence analysis, the MELproducing strain was assigned to P. antarctica while the four MEL- and CL-producing strains were assigned to P. hubeiensis. These results demonstrate that MALDI-TOF/MS is a rapid and reliable tool to detect BS molecules in crude extracts of broth cultures to screen for glycolipid-type BS producers.
- Subjects
GLYCOLIPIDS; BIOSURFACTANTS; MATRIX-assisted laser desorption-ionization; LIPID metabolism; CELL culture
- Publication
Journal of Oleo Science, 2019, Vol 68, Issue 12, p1287
- ISSN
1345-8957
- Publication type
Article
- DOI
10.5650/jos.ess19177