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- Title
Self-assembling nanoprobes that display off/on <sup>19</sup>F nuclear magnetic resonance signals for protein detection and imaging.
- Authors
Takaoka, Yousuke; Sakamoto, Takashi; Tsukiji, Shinya; Narazaki, Michiko; Matsuda, Tetsuya; Tochio, Hidehito; Shirakawa, Masahiro; Hamachi, Itaru
- Abstract
Magnetic resonance imaging (MRI) is one of the most promising techniques for the non-invasive visualization of biomarkers and biologically relevant species, both in vivo and ex vivo. Although 1H MRI with paramagnetic contrast agents, such as Gd3+ complexes and iron oxide, is widely used, it often suffers from low contrast because of the large background signals caused by the abundant distribution of protons in biological samples. Here we report the use of supramolecular organic nanoparticles to detect specific proteins by 19F-based MRI in an off/on mode. In NMR spectroscopy these designed probes are silent when aggregated, but in the presence of a target protein they disassemble to produce a sharp signal. This ‘turn-on’ response allowed us to visualize clearly the protein within live cells by 19F MRI and construct an in-cell inhibitor assay. This recognition-driven disassembly of nanoprobes for a turn-on 19F signal is unprecedented and may extend the use of 19F MRI for specific protein imaging.
- Subjects
MOLECULAR self-assembly; NUCLEAR magnetic resonance; BIOMARKERS; PARAMAGNETIC contrast media; IRON oxides; PROTON scattering; NANOPARTICLES; MAGNETIC resonance imaging; SPECTRUM analysis
- Publication
Nature Chemistry, 2009, Vol 1, Issue 7, p557
- ISSN
1755-4330
- Publication type
Article
- DOI
10.1038/nchem.365