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- Title
Investigating the effect of the inhibitory peptide on L.monocytogenes cell invasion: an in silico and in vitro study.
- Authors
Shivaee, Ali; Bahonar, Sara; Goudarzi, Mehdi; Hematian, Ali; Hajikhani, Bahareh; Sadeghi Kalani, Behrooz
- Abstract
Aims: L.monocytogenes monocytogenes is an omnipresent bacterium that causes a fatal food-borne illness, listeriosis. The connection of this bacterium to E-cadherin through internalin A plays a significant role in the internalization of the bacteria. In this study, this interaction has been investigated for the design of an inhibitory peptide. Methods: The interaction of the proteins involved in the entry of bacteria was evaluated by molecular docking. According to their interactions, an inhibitory peptide was designed to bind to internalin A by server peptiderive. Its effects on L.monocytogenes invasion on the Caco-2 cell line and biofilm formation were also assessed. Findings: Docking results showed that the peptide has a high affinity for binding to Internalin A. The synthesized peptide at a concentration of 64 µg/ml inhibited 80% of the invasion of L.monocytogenes into the Caco-2 cell line. Furthermore, the studied peptide at the highest concentration had a slight inhibitory effect on biofilm formation. Conclusion: These results reveal that short polypeptides can impede the invasion of target cells by L. monocytogenes in vitro and could be advantageous as restoring agents in vivo.
- Subjects
PEPTIDES; IN vitro studies; MOLECULAR docking; PROTEIN-protein interactions; POLYPEPTIDES
- Publication
Gut Pathogens, 2023, Vol 15, Issue 1, p1
- ISSN
1757-4749
- Publication type
Article
- DOI
10.1186/s13099-023-00576-7