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- Title
The α-Glucosidase Inhibiting Isoflavones Isolated from Belamcanda chinensis Leaf Extract.
- Authors
Chongming Wu; Jingyao Shen; Pingping He; Yan Chen; Liang Li; Liang Zhang; Ye Li; Yujuan Fu; Rongji Dai; Weiwei Meng; Yulin Deng
- Abstract
The dried rhizome of Belamcanda chinensis is an important Chinese traditional medicine used for the treatment of inflammation and many other disorders. Previously, we reported the hypo- and antihyper-glycemic effects of the aqueous leaf extract of B. chinensis (BCL) and identified the isoflavones as its principal active fraction. In the present study, the α-glucosidase inhibitory effect of BCL and its rough isoflavone preparation (BIF) was tested in vitro and in vivo. Thirteen isoflavones were isolated from BCL and their α-glucosidase inhibitory activity was screened in vitro. The results showed that BCL (500 and 1000 mg/kg) and BIF (250 and 500 mg/kg) greatly inhibited the increase in blood glucose level after 5 g/kg starch loading in normal mice. Six out of the thirteen isoflavones (swertisin, 2"-O-rhamnosylswertisin, genistein, genistin, mangiferin and daidzin) exhibited strong α-glucosidase inhibitory activity in vitro. HPLC analysis showed that swertisin was the most abundant isoflavone in BCL accounting for 1.24% of BCL, 7.44% of BIF, and 11.24% of the total isoflavone fraction of BCL, respectively. These results demonstrate that BCL possesses significant α-glucosidase inhibitory activity and swertisin may be the principal active component of BCL in α-glucosidase inhibition.
- Subjects
GLUCOSIDASE inhibitors; ISOFLAVONES; BLACKBERRIES; PLANT extracts; CHINESE medicine; INFLAMMATION; HYPERGLYCEMIA; DIABETES
- Publication
Records of Natural Products, 2012, Vol 6, Issue 2, p110
- ISSN
1307-6167
- Publication type
Article