We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Efecto de la suplementación con ciclodextrinas cargadas con colesterol al semen sexado bovino post-descongelación para su uso en producción in vitro de embriones.
- Authors
Ferré, L.; Grötter, L.; Fresno, C.; Cattaneo, L.
- Abstract
Bovine sperm have a low cholesterol/phospholipids ratio in the sperm plasma membrane. This characteristic is important due to cholesterol plays a key role in maintaining the structure and function of sperm membrane after freezing, and therefore, increasing sperm survival. This situation could be amplified in case of sexed semen. The aim of this study is to stabilize the plasma membranes of sperm sexed by adding cholesterol-loaded cyclodextrins (CLC) post-thaw and consequently extend their functionality, increase their ability to fertilize oocytes and produce embryos in vitro. Sexed sperm were treated with CLC at different concentrations and incubated for 15 minutes before evaluating different motility parameters by a computerized system (CASA) and fertilize oocytes in vitro. Sperm treated with 3 mg of methyl-β-cyclodextrin saturated cholesterol for each 120x106 of sperm, showed higher motility parameters and remained high during the analyzed period. The concentration of 3 mg of CLC also increased cleavage rate, early (MOR/eBL), late (BL) and total embryo development.
- Publication
Archivos de Zootecnia, 2018, Vol 67, Issue 259, p332
- ISSN
0004-0592
- Publication type
Article
- DOI
10.21071/az.v67i259.3788