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- Title
Expression and clinical significance of cytochrome c oxidase subunit IV in colorectal cancer patients.
- Authors
Kun Zhang; Yibing Chen; Xiaojun Huang; Ping Qu; Qiuzhong Pan; Lin Lü; Shanshan Jiang; Tingitng Ren; Haichuan Su; Zhang, Kun; Chen, Yibing; Huang, Xiaojun; Qu, Ping; Pan, Qiuzhong; Lü, Lin; Jiang, Shanshan; Ren, Tingitng; Su, Haichuan
- Abstract
<bold>Introduction: </bold>Previous studies have demonstrated that the expression of cytochrome c oxidase (COX) subunits encoded by mitochondrial DNA is elevated in colorectal cancer (CRC). However, the expression of nuclear DNA-encoded COX IV and its clinical significance have not yet been investigated in CRC.<bold>Material and Methods: </bold>We examined COX IV expression in paired CRC samples (cancer and pericancerous tissues) by quantitative real-time polymerase chain reaction (qPCR), Western blot and immunohistochemical staining and analyzed its clinical significance.<bold>Results: </bold>qPCR and Western blot analyses showed that COX IV expression was significantly elevated at both the mRNA (p = 0.05) and protein levels in CRC tissue samples when compared with those in paired pericancerous tissues. Immunohistochemistry also revealed that COX IV expression was significantly increased in CRC tissues (p < 0.001). Association analyses showed that there was no significant association between COX IV expression and clinical parameters of CRC patients except for gender (p = 0.017). Moreover, we did not find any association between COX IV expression and overall survival or recurrence-free survival of CRC patients. Further analysis showed no significant relationship between the expression of COX IV and proliferating cell nuclear antigen (PCNA), a marker of cell proliferation.<bold>Conclusions: </bold>Our findings suggest that elevated COX IV expression may play an important role in colorectal carcinogenesis, but not in progression, which warrants further investigation in future studies.
- Subjects
CYTOCHROME oxidase; COLON cancer patients; MITOCHONDRIAL DNA; CLINICAL medicine; POLYMERASE chain reaction
- Publication
Archives of Medical Science, 2016, Vol 12, Issue 1, p68
- ISSN
1734-1922
- Publication type
journal article
- DOI
10.5114/aoms.2016.57581