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- Title
NAADP mediates ATP-induced Ca<sup>2+</sup> signals in astrocytes
- Authors
Barceló-Torns, Miquel; Lewis, Alexander M.; Gubern, Albert; Barneda, David; Bloor-Young, Duncan; Picatoste, Fernado; Churchill, Grant C.; Claro, Enrique; Masgrau, Roser
- Abstract
Abstract: Intracellular Ca2+ signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca2+ in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca2+ responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes.
- Subjects
ASTROCYTES; NUCLEOTIDES; ADENOSINE triphosphate; CALCIUM; LYSOSOMES; CYCLIC adenylic acid; PHENYLALANINE; BRADYKININ
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2300
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.05.062