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- Title
In vitro regulation of circadian phosphorylation rhythm of cyanobacterial clock protein KaiC by KaiA and KaiB
- Authors
Nakajima, Masato; Ito, Hiroshi; Kondo, Takao
- Abstract
Abstract: Biochemical circadian oscillation of KaiC phosphorylation, by mixing three Kai proteins and ATP, has been proven to be the central oscillator of the cyanobacterial circadian clock. In vivo, the intracellular levels of KaiB and KaiC oscillate in a circadian fashion. By scrutinizing KaiC phosphorylation rhythm in a wide range of Kai protein concentrations, KaiA and KaiB were found to be “parameter-tuning” and “state-switching” regulators of KaiC phosphorylation rhythm, respectively. Our results also suggest a possible entrainment mechanism of the cellular circadian clock with the circadian variation of intracellular levels of Kai proteins.
- Subjects
EFFECT of chemicals on circadian rhythms; PHOSPHORYLATION; ADENOSINE triphosphate; CYANOBACTERIA; GENETIC regulation; PROTEIN analysis
- Publication
FEBS Letters, 2010, Vol 584, Issue 5, p898
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.01.016