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- Title
A soluble and active form of Wnt-3a protein is involved in myogenic differentiation after cholesterol depletion
- Authors
Portilho, Débora M.; Martins, Eliane R.; Costa, Manoel L.; Mermelstein, Cláudia S.
- Abstract
Abstract: Cholesterol is one of the major lipids of plasma membranes. Recently, we have shown that cholesterol depletion by methyl-β-cyclodextrin (MβCD) induces the activation of the Wnt/β-catenin pathway and enhances myogenic differentiation. Here, we show that MβCD-conditioned media accelerates myogenesis in a similar way as MβCD does, suggesting that the effects induced by MβCD could be caused by soluble factors present in the culture medium. Soluble Wnt-3 protein is significantly enhanced in MβCD-conditioned medium. Wnt-3a-enriched media induces myogenesis as much as MβCD does, whereas Wnt-5a-enriched media inhibits. We suggest that Wnt-3a is involved in the myogenic induction observed after cholesterol depletion.
- Subjects
CHOLESTEROL; CELL membranes; MYOGENESIS; BIOMOLECULES
- Publication
FEBS Letters, 2007, Vol 581, Issue 30, p5787
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.11.047