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- Title
Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum
- Authors
Röhrich, René C.; Englert, Nadine; Troschke, Katrin; Reichenberg, Armin; Hintz, Martin; Seeber, Frank; Balconi, Emanuela; Aliverti, Alessandro; Zanetti, Giuliana; Köhler, Uwe; Pfeiffer, Matthias; Beck, Ewald; Jomaa, Hassan; Wiesner, Jochen
- Abstract
Abstract: In the malaria parasite Plasmodium falciparum isoprenoid precursors are synthesised inside a plastid-like organelle (apicoplast) by the mevalonate independent 1-deoxy-d-xylulose-5-phosphate (DOXP) pathway. The last reaction step of the DOXP pathway is catalysed by the LytB enzyme which contains a [4Fe–4S] cluster. In this study, LytB of P. falciparum was shown to be catalytically active in the presence of an NADPH dependent electron transfer system comprising ferredoxin and ferredoxin-NADP+ reductase. LytB and ferredoxin were found to form a stable protein complex. These data suggest that the ferredoxin/ferredoxin-NADP+ reductase redox system serves as the physiological electron donor for LytB in the apicoplast of P. falciparum.
- Subjects
PLASMODIUM falciparum; MALARIA; PROTOZOAN diseases; ENZYMES
- Publication
FEBS Letters, 2005, Vol 579, Issue 28, p6433
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.10.037