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- Title
Rapid Detection of BF Haplotypes by a Semi-nested Polymerase Chain Reaction, which Causes Resistance/Susceptibility to Marek’s Disease in Chicken.
- Authors
Jin, Y.-C.; Wei, P.; Wei, X.-X.; Zhao, Z.-Y.; Li, Y.
- Abstract
A semi-nested polymerase chain reaction (snPCR) assay was developed for the rapid detection of resistant/susceptible BF haplotypes to Marek’s disease (MD) using the cDNA samples from peripheral blood leucocytes, liver, spleen and heart from Xiayan homozygous chickens: A11, C23, D8 and D12 (resistant to MD), A5 and B21 (susceptible to MD). The snPCR was utilized to span alternative splicing-out of the sequence encoding the second segment of the cytoplasmic part of the mature BF molecules (exon 7). This alternative exon 7 splice variant was detected in BF*A5 and BF*B21 (susceptible to MD), but not in the MD-resistant BF*A11, BF*C23, BF*D8 and BF*D12 haplotypes, suggesting a potential role of exon 7 for the detection of resistant/susceptible BF haplotypes to MD.
- Subjects
POLYMERASE chain reaction; DNA polymerases; BLOOD cells; LEUCOCYTES; KILLER cells
- Publication
Scandinavian Journal of Immunology, 2010, Vol 72, Issue 2, p94
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2010.02415.x