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- Title
Surface Expression of Transglutaminase 2 by Dendritic Cells and its Potential Role for Uptake and Presentation of Gluten Peptides to T Cells.
- Authors
Ráki, M.; Schjetne, K. W.; Stamnaes, J.; Molberg, Ø.; Jahnsen, F. L.; Issekutz, T. B.; Bogen, B.; Sollid, L. M.
- Abstract
Celiac disease is a chronic small intestinal inflammation driven by gluten-reactive T cells of the intestinal mucosa. These T cells are HLA-DQ2 or -DQ8 restricted, and predominantly recognize gluten peptides that are deamidated by the enzyme transglutaminase 2 (TG2). Our recent results strongly suggest that duodenal CD11c+ dendritic cells (DC) are directly involved in T cell activation in the celiac lesion. The aim of this study was to investigate whether surface-associated TG2 could be involved in receptor-mediated endocytosis of gluten peptides, a process that may contribute to the preferential recognition of deamidated peptides. We found that both monocyte-derived DC and local CD11c+ DC in the duodenal mucosa expressed cell surface-associated TG2. As phenotypic characterization of CD11c+ DC in the celiac lesion suggests that these cells may be derived from circulating monocytes, we used monocyte-derived DC in functional in vitro studies. Using a functional T cell assay, we obtained evidence that cell surface-associated TG2 is endocytosed by monocyte-derived DC. However, we were unable to obtain evidence for a role of surface TG2 in the loading and subsequent generation of deamidated gluten peptides in these cells.
- Subjects
TRANSGLUTAMINASES; DENDRITIC cells; PEPTIDES; T cells; CELIAC disease; INTESTINAL mucosa
- Publication
Scandinavian Journal of Immunology, 2007, Vol 65, Issue 3, p213
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2006.01881.x