We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Metabolic study of ginsenoside Rg3 and glimepiride in type 2 diabetic rats by liquid chromatography coupled with quadrupole‐Orbitrap mass spectrometry.
- Authors
Yang, Di; Li, Xue; Fu, Yunhua; Tao, Xingyu; Zheng, Fei; Yu, Jiangbo; Yue, Hao; Dai, Yulin
- Abstract
Rationale: Ginsenoside Rg3 and glimepiride have been applied to treat type 2 diabetes (T2DM) because of their good hypoglycemic effects. In this study, the effects of ginsenoside Rg3 acting synergistically with glimepiride were investigated in liver microsomes from rats with type 2 diabetes. Methods: An in vitro incubation system with normal rat liver microsomes (RLM) and type 2 diabetic rat liver microsomes (TRLM) was developed. The system also included two experimental groups consisting of RLM and TRLM pretreated with ginsenoside Rg3 and glimepiride (named the RLMR and TRLMR groups, respectively). The metabolism in the different groups was analyzed by ultra‐performance liquid chromatography coupled with quadrupole‐orbitrap mass spectrometry (UPLC/Q‐Orbitrap MS). Results: The results showed that the concentration of glimepiride increased in RLM and TRLM after treatment with ginsenoside Rg3. Five metabolites (M1–M5) of glimepiride were found, and they were named 3N‐hydroxyglimepiride, hydroxyglimepiride, 1,2‐epoxy ether‐3‐hydroxyglimepiride, 1N‐hydroxyglimepiride and 1N,2C,S,O,O‐epoxy ether‐3‐hydroxyglimepiride. The metabolite of ginsenoside Rg3 was ginsenoside Rh2. Conclusions: An in vitro incubation system with RLM and TRLM was developed. The system revealed pathways that produce glimepiride metabolites. Ginsenoside Rg3 may inhibit the activity of cytochrome P450 enzymes in vitro. The present study showed that ginsenoside Rg3 and glimepiride may be combined for the treatment of T2DM.
- Subjects
MASS spectrometry; LIQUID chromatography; LIVER microsomes; QUADRUPOLE ion trap mass spectrometry; TYPE 2 diabetes; LIQUID chromatography-mass spectrometry; CYTOCHROME P-450; QUADRUPOLE mass analyzers
- Publication
Rapid Communications in Mass Spectrometry: RCM, 2021, Vol 35, Issue 11, p1
- ISSN
0951-4198
- Publication type
Article
- DOI
10.1002/rcm.9083