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- Title
Use of the P Gene to Genotype Human Metapneumovirus Identifies 4 Viral Subtypes.
- Authors
Mackay, Ian M.; Bialasiewicz, Seweryn; Waliuzzaman, Zubair; Chidlow, Glenys R.; Fegredo, David C.; Laingam, Somprasong; Adamson, Penny; Harnett, Gerald B.; Rawlinson, William; Nissen, Michael D.; Sloots, Theo P.
- Abstract
This study, conducted during 2001-2003, undertook the screening of patients with acute infectious respiratory-tract disease. A random selection of positive specimens was used for sequencing studies of the human metapneumovirus (hMPV) nucleoprotein gene and the phosphoprotein (P) gene. Australian and international sequences were compared, and a global classification scheme was developed. The hMPV P gene was an ideal molecular target for the detection and genotyping of hMPV. The region contained conserved sequences for reverse-transcriptase-polymerase chain-reaction primers and adequate variability to permit the accurate genotyping of the virus into 2 main lineages and 4 sublineages. Establishing viral identity is essential for the linking of genotype and disease severity.
- Subjects
RESPIRATORY infections; RESPIRATORY diseases; COMMUNICABLE diseases; NUCLEOPROTEINS; GENETIC polymorphisms; VIRUSES
- Publication
Journal of Infectious Diseases, 2004, Vol 190, Issue 11, p1913
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/425013