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- Title
An engineered eukaryotic protein glycosylation pathway in Escherichia coli.
- Authors
Valderrama-Rincon, Juan D; Fisher, Adam C; Merritt, Judith H; Fan, Yao-Yun; Reading, Craig A; Chhiba, Krishan; Heiss, Christian; Azadi, Parastoo; Aebi, Markus; DeLisa, Matthew P
- Abstract
We performed bottom-up engineering of a synthetic pathway in Escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. The glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, we successfully transferred glycans to eukaryotic proteins.
- Subjects
ESCHERICHIA coli; GLYCOSYLATION; GLYCANS; ASPARAGINE synthetase; BIOSYNTHESIS; GLYCOSYLTRANSFERASES; OLIGOSACCHARYLTRANSFERASE
- Publication
Nature Chemical Biology, 2012, Vol 8, Issue 5, p434
- ISSN
1552-4450
- Publication type
Article
- DOI
10.1038/nchembio.921