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- Title
The Effect of Fucoidan from the Brown Alga Fucus evanescence on the Activity of α-N-Acetylgalactosaminidase of Human Colon Carcinoma Cells.
- Authors
Bakunina, Irina; Malyarenko, Olesya; Ermakova, Svetlana; Chadova, Oksana
- Abstract
α-<italic>N</italic>-acetylgalactosaminidase (EC 3.2.1.49) (alpha-NaGalase) catalyzes the hydrolysis of <italic>N</italic>-acetamido-2-deoxy-α-d-galactoside residues from non-reducing ends of various complex carbohydrates and glycoconjugates. It is known that human cancer cells express an alpha-NaGalase, which accumulates in the blood plasma of patients. The enzyme deglycosylates the Gc protein-derived macrophage activating factor (GcMAF) and inhibits macrophage activity acting as an immunosuppressor. The high specific activity 0.033 ± 0.002 μmol mg−1 min−1 of the enzyme was found in human colon carcinoma cells DLD-1. The alpha-NaGalase of DLD-1 cells was isolated and biochemical characterized. The enzyme exhibits maximum activity at pH 5.2 and temperature 55 °C. The <italic>K</italic>m is 2.15 mM, <italic>V</italic>max–0.021 μmol min−1 mL−1, <italic>k</italic>cat–1.55 min−1 and <italic>k</italic>cat/<italic>K</italic>m–0.72 min−1 mM−1 at 37 °C, pH 5.2. The effects of fucoidan from the brown alga <italic>Fucus evanescence</italic> on the activity of alpha-NaGalase in human colon carcinoma DLD-1 cells and on the biosynthesis of this enzyme were investigated. It was shown that fucoidan did not inhibit free alpha-NaGalase, however, it reduced the expression of the enzyme in the DLD-1 cells at IC50 73 ± 4 μg mL−1.
- Subjects
BROWN algae; FUCUS; ACETYLAMINOFLUORENE; MACROPHAGE activating factors; GLYCOSIDASES
- Publication
Marine Drugs, 2018, Vol 16, Issue 5, p155
- ISSN
1660-3397
- Publication type
Article
- DOI
10.3390/md16050155