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- Title
GelMA promotes inner ear organoidogenesis by regulating Mmp-mediated extracellular matrix remodeling.
- Authors
Li, N.; Zhang, Z.; Zhang, L.; Wu, D.; Li, Z.; Xu, C.; Sun, Q.; Lu, Y.; Zhou, Y.; Hu, Y.; Tan, F.; Qi, J.; Chai, R.
- Abstract
Permanent damage and loss of inner ear hair cells due to genetic mutations or external factors such as drugs and noise are the main causes of irreversible hearing loss. Inner ear organoids can provide an in vitro model for studying the underlying mechanisms of injury and for developing new therapeutic approaches. However, there is lack of protocols for the rapid and efficient establishment of inner ear organoids. In this study, we established a novel method for creating inner ear organoids using a synthetic GelMA hydrogel culture system to promote the spontaneous aggregation and assembly of inner ear stem cells in order to rapidly form organoids, and we found that the extracellular matrix undergoes extensive and rapid remodeling during self-assembly. The expression and activity of the Mmp family of extracellular matrix degradation enzymes, especially matrix metalloproteinase 9 (Mmp9), were increased in inner ear organoids in the hydrogel culture system, and inhibition of Mmps significantly inhibited the formation of inner ear organoids. Our study is the first to combine analysis of the extracellular matrix with inner ear organogenesis and provides a rapid and efficient method to model inner ear organoids.
- Subjects
POLAND; TISSUE engineering; PHARMACEUTICAL gels; CONFERENCES &; conventions; INNER ear; MATRIX metalloproteinases; EXTRACELLULAR matrix
- Publication
Journal of Hearing Science, 2024, Vol 14, Issue 3, p126
- ISSN
2083-389X
- Publication type
Article