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- Title
全氟辛酸 (PFOA) 对菲律宾蛤仔血淋巴的毒性效应 研究.
- Authors
龚秀琼; 周扬; 李风铃; 郭萌萌; 耿倩倩; 谭志军; 姚琳; 江艳华; 曲梦
- Abstract
Perfluorooctanoic acid (PFOA) is an emerging contaminant in the marine environment. Bivalves are important indicator species in marine environment, which are commonly used to assess pollutants toxicity and monitor the ecological environment. Hemocytes are the main component of immune system in bivalves, and are often used to indicate the immunotoxicity of pollutants. In this study, PFOA bioaccumulation in hemolymph and immune-related indicators, including total hemocyte count (THC), hemocyte viability, apoptosis, phagocytosis, reactive oxygen species (ROS), and non-specific esterase activity in Ruditapes philippinarum, were measured. We intended to systematically assess PFOA immunotoxicity from three perspectives, including immune organs, immune functions, and immune factors. The results indicated that PFOA accumulated significantly in the hemolymph. After 14 days of PFOA exposure, the contents were (122.5 ±5.35), (149.38 ±0.52), and (157.23 ±4.65) ng • mL- 1 in hemolymph from treatment groups of PFOA-L, PFOA-M, and PFOA-H, respectively. After a 7-day sea water recovery period, the accumulation of PFOA in three treatment groups were still significantly higher than the control group, and were (3.33±0.16), (5.42±0.08), (6.20±0.20) ng·mL-1, respectively. We further discovered that PFOA exposure triggered significant changes in various physiological indexes and immune functions in clam hemocytes. PFOA reduced the THC of clams in a dose-dependent manner, changed the proportions of different types of hemocytes. Meanwhile, PFOA also caused a decline in phagocytic ability, and increase in the levels of ROS and non-specific esterase. In addition, PFOA exposure also significantly altered the expression levels of immune-related genes. After a 7-day sea water recovery period, the above indicators had not returned to the level of the control group. It was evident that PFOA exposure triggered significant immunotoxic effects in R. philippinarum. This study provides scientific basis for revealing the potential risk of PFOA to the marine ecosystem and scientific evidence for screening bioindicators for per- and polyfluoroalkyl substances (PFASs) pollution in the oceans.
- Publication
Asian Journals of Ecotoxicology, 2024, Vol 19, Issue 4, p270
- ISSN
1673-5897
- Publication type
Article
- DOI
10.7524/AJE.1673-5897.20240228003