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- Title
Subcutaneous Injections of the Mannose-Sensitive Hemagglutination Pilus Strain of Pseudomonas aeruginosa Stimulate Host Immunity, Reduce Bladder Cancer Size and Improve Tumor Survival in Mice.
- Authors
Li, Tao; Yang, Li; Fu, Sheng-jun; Xiao, Er-long; Yuan, Xuan; Lu, Jian-zhong; Ma, Bao-liang; Shi, Ting-kai; Wang, Zhi-ping
- Abstract
We wished to evaluate the effects of Pseudomonas aeruginosa (mannose-sensitive hemagglutination pilus strain, PA-MSHA) as an immunostimulating and anti-tumor agent for treatment of bladder cancer. Immunostimulating effects were assessed by the in vitro proliferation assay of murine splenic lymphocytes. Anti-tumor effects were studied in a subcutaneous tumor model established in female C57BL/6 mice using the MB49 bladder cell line. These mice received subcutaneous injections of normal saline (control group) or PA-MSHA (high, medium, or low dose, respectively, 1.6-2.0 × 10, 3.2- .0 × 10, 6.4-8.0 × 10 CFU/ml) twice a week for 3 weeks. Mice survival, tumor volume, vascular endothelial growth factor (VEGF) expression, microvessel density (MVD), serum levels of TNF-α and IFN-γ, and blood CD4 /CD8 counts were the study outcomes. We observed that PA-MSHA promoted the growth of splenic lymphocytes in vitro. In the murine tumor model, PA-MSHA prolonged mice survival and reduced tumor growth. Furthermore, VEGF and MVD were also diminished by PA-MSHA. Mice that received high and medium dose of PA-MSHA had significantly higher serum levels of IFN-γ and TNF-α (days 21 and 28), and higher levels of CD4 /CD8 cells (days 21 and 28). In conclusion, PA-MSHA exerts beneficial effects on increasing proliferation of murine splenic lymphocytes in vitro and inhibits the growth of bladder tumor in a murine model. Therefore, PA-MSHA may be useful an immunostimulating and anti-tumor agent for bladder cancer therapy.
- Subjects
HEMAGGLUTINATION tests; PSEUDOMONAS aeruginosa; BLADDER cancer; LABORATORY mice; IMMUNOLOGICAL adjuvants; CELL proliferation
- Publication
Cell Biochemistry & Biophysics, 2015, Vol 73, Issue 1, p245
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-015-0611-y