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- Title
Autophagy Protects MC3T3-E1 Cells upon Aluminum-Induced Apoptosis.
- Authors
Yang, Xu; Zhang, Jian; Ji, Qiang; Wang, Fan; Song, Miao; Li, Yanfei
- Abstract
Aluminum (Al) exposure has adverse effects on osteoblasts, and the effect might be through autophagy-associated apoptosis. In this study, we showed that aluminum trichloride (AlCl3) could induce autophagy in MC3T3-E1 cells, as demonstrated by monodansylcadaverine (MDC) staining and the expressions of the ATG3, ATG5, and ATG9 genes. We found AlCl3 inhibited MC3T3-E1 cell survival rate and caused apoptosis, as evidenced by CCK-8 assay, Annexin V/PI double staining, and increased expressions of Bcl-2, Bax, and Caspase-3 genes. In addition, increased autophagy induced by rapamycin further attenuated the MC3T3-E1 cell apoptosis rate after AlCl3 exposure. These results support the hypothesis that autophagy plays a protective role in impeding apoptosis caused by AlCl3. Activating autophagy may be a strategy for treatment of Al-induced bone disease.
- Subjects
AUTOPHAGY; TOXICOLOGY of aluminum; APOPTOSIS; TREATMENT of bone diseases; BIOLOGICAL assay
- Publication
Biological Trace Element Research, 2018, Vol 185, Issue 2, p433
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-018-1264-7