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- Title
WSF-P-1, a novel AMPK activator, promotes adiponectin multimerization in 3T3-L1 adipocytes.
- Authors
Wang, Yao; Zhang, Yudian; Li, Zhen; Wang, Yunyun; Peng, Han; Rui, Jian; Wang, Shifa; Zhang, Zhijie
- Abstract
Adiponectin, an adipokine with insulin-sensitizing effect, is secreted from adipocytes into circulation as high, medium, and low molecular weight forms (HMW, MMW, and LMW). The HMW adiponectin oligomers possess the most potent insulin-sensitizing activity. WSF-P-1(N-methyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4α-methanonaphthalen-7-amine) is derived from natural sesquiterpene longifolene by chemical modifications. We found that WSF-P-1 activates AMPK in both 3T3-L1 adipocytes and 293T cells in this study. Activation of AMPK by WSF-P-1 promotes the assembly of HMW adiponectin and increases the HMW/total ratio of adiponectin in 3T3-L1 adipocytes. We demonstrated that the Ca2+-dependent CaMKK signaling pathway is involved in WSF-P-1-induced AMPK activation and adiponectin multimerization. WSF-P-1 also activates GLUT1-mediated glucose uptake in 3T3-L1 adipocytes, making it a potential drug candidate for the treatment of type 2 diabetes, obesity, and other obesity-related metabolic diseases. WSF-P-1 activates AMPK via the Ca2+-CaMKK signaling pathway and promotes adiponectin multimerization and glucose uptake in 3T3-L1 adipocytes.
- Subjects
ADIPONECTIN; TYPE 2 diabetes; INSULIN resistance; THERAPEUTICS
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 8, p1529
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2017.1336923