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- Title
DNA separation with low-viscosity sieving matrix on microfabricated polycarbonate microfluidic chips.
- Authors
Mei-Ying Ye; Xue-Feng Yin; Zhao-Lun Fang
- Abstract
Microfluidic devices have been fabricated on polycarbonate (PC) substrates by use of a hot embossing method using a silicon master template. By adding auxiliary lines around the functional channel on the silicon master, its lifetime was significantly prolonged and the bonding strength of the PC cover plate to the microfluidic chip was greatly improved. More than 300 polycarbonate microfluidic chips have been replicated with the same silicon mold. CE separation of F X-174/HaeIII DNA restriction fragments, with high resolution efficiency and good reproducibility, was achieved on these devices using the low-viscosity sieving matrix HPMC-50. Temperature was found to have a significant effect on separation efficiency.
- Subjects
POLYCARBONATES; SEPARATION (Technology); SILICON; VISCOSITY; CHEMICAL bonds
- Publication
Analytical & Bioanalytical Chemistry, 2005, Vol 381, Issue 4, p820
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-004-2988-0