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- Title
Aptamers against Cells Overexpressing Glypican 3 from Expanded Genetic Systems Combined with Cell Engineering and Laboratory Evolution.
- Authors
Zhang, Liqin; Yang, Zunyi; Le Trinh, Thu; Teng, I ‐ Ting; Wang, Sai; Bradley, Kevin M.; Hoshika, Shuichi; Wu, Qunfeng; Cansiz, Sena; Rowold, Diane J.; McLendon, Christopher; Kim, Myong ‐ Sang; Wu, Yuan; Cui, Cheng; Liu, Yuan; Hou, Weijia; Stewart, Kimberly; Wan, Shuo; Liu, Chen; Benner, Steven A.
- Abstract
Laboratory in vitro evolution (LIVE) might deliver DNA aptamers that bind proteins expressed on the surface of cells. In this work, we used cell engineering to place glypican 3 (GPC3), a possible marker for liver cancer theranostics, on the surface of a liver cell line. Libraries were then built from a six-letter genetic alphabet containing the standard nucleobases and two added nucleobases (2-amino-8 H-imidazo[1,2- a][1,3,5]triazin-4-one and 6-amino-5-nitropyridin-2-one), Watson-Crick complements from an artificially expanded genetic information system (AEGIS). With counterselection against non-engineered cells, eight AEGIS-containing aptamers were recovered. Five bound selectively to GPC3-overexpressing cells. This selection-counterselection scheme had acceptable statistics, notwithstanding the possibility that cells engineered to overexpress GPC3 might also express different off-target proteins. This is the first example of such a combination.
- Subjects
APTAMERS; GLYPICANS; PROTEINS; LIVER cancer; CELL lines; GENETIC overexpression
- Publication
Angewandte Chemie, 2016, Vol 128, Issue 40, p12560
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201605058