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- Title
Rapid enzymatic method for measurement of serum flucytosine levels.
- Authors
Washburn, R G; Klym, D M; Kroll, M H; Bennett, J E
- Abstract
Creatinine iminohydrolase (EC 3.5.4.21) quantitatively releases ammonia from flucytosine (5FC) as well as from creatinine. Using 39 sera from eight patients receiving the combination of amphotericin B with 5FC, we demonstrated that this rapid enzymatic reaction provides a valid measure of 5FC levels. The correlation coefficients (r), comparing this method with 5FC bioassay and high-pressure liquid chromatography measurements, were 0.78 and 0.87, respectively. This technique offers a precise alternative to the currently employed methods of measuring 5FC levels. In contrast to other methods, this enzymatic technique is rapid, simple, inexpensive, and has the potential for widespread availability.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 1986, Vol 17, Issue 4, p673
- ISSN
0305-7453
- Publication type
journal article