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- Title
A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides.
- Authors
Liu, Ke; Zhou, Youzhi; Zhang, Jinsong; Chu, Jianlin; Zhang, Yewei; He, Bingfang
- Abstract
Objective: To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining. Results: BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2′-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61-84%. Conclusion: An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.
- Subjects
PYRIMIDINE nucleosides; NUCLEOSIDE synthesis; BREVIBACILLUS brevis; THYMIDINE; THERMAL stability
- Publication
Biotechnology Letters, 2017, Vol 39, Issue 12, p1903
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-017-2423-1