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- Title
In vitro antifungal activity of synthetic dsRNA molecules against two pathogens of banana, Fusarium oxysporum f. sp. cubense and Mycosphaerella fijiensis.
- Authors
Mumbanza, Francis M; Kiggundu, Andrew; Tusiime, Geoffrey; Tushemereirwe, Wilberforce K; Niblett, Chuck; Bailey, Anna
- Abstract
BACKGROUND A key challenge for designing RNAi-based crop protection strategies is the identification of effective target genes in the pathogenic organism. In this study, in vitro antifungal activities of a set of synthetic double-stranded RNA molecules on spore germination of two major pathogenic fungi of banana, Fusarium oxysporum Schlecht f. sp. cubense WC Snyder & HN Hans (Foc) and Mycosphaerella fijiensis Morelet (Mf) were evaluated. RESULTS All the tested synthetic dsRNAs successfully triggered the silencing of target genes and displayed varying degrees of potential to inhibit spore germination of both tested banana pathogens. When Foc dsRNAs were applied to Foc spores, inhibition ranged from 79.8 to 93.0%, and from 19.9 to 57.8% when Foc dsRNAs were applied to Mf spores. However, when Mf dsRNAs were applied on Mf spores, inhibition ranged from 34.4 to 72.3%, and from 89.7 to 95.9% when Mf dsRNAs were applied to Foc spores. CONCLUSION The dsRNAs for adenylate cyclase, DNA polymerase alpha subunit and DNA polymerase delta subunit showed high levels of spore germination inhibition during both self- and cross-species tests, making them the most promising targets for RNA-mediated resistance in banana against these fungal pathogens. © 2013 Society of Chemical Industry
- Subjects
ANTIFUNGAL agents; DOUBLE-stranded RNA; BANANAS; FUSARIUM oxysporum; MYCOSPHAERELLA; PLANT protection; DNA polymerases
- Publication
Pest Management Science, 2013, Vol 69, Issue 10, p1155
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.3480