We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Prevalence of Nitrosomonas cluster 7 populations in the ammonia-oxidizing community of a submerged membrane bioreactor treating urban wastewater under different operation conditions.
- Authors
Cerrone, F.; Poyatos, J.; Molina-Muñoz, M.; Cortés-Lorenzo, C.; González-López, J.; Rodelas, B.
- Abstract
A pilot-scale ultrafiltration membrane bioreactor (MBR) was used for the aerobic treatment of urban wastewater in four experimental stages influenced by seasonal temperature and different sets of operation conditions. The structure of the ammonia-oxidizing bacteria (AOB) community was profiled by temperature gradient gel electrophoresis (TGGE), based on the amplification and separation of partial ammonia-monoxygenase subunit A ( amoA) genes. Canonical correspondence analysis revealed that temperature, hydraulic retention time and percentage of ammonia removal had a significant effect on the fingerprints of AOB communities. Phylogenetic analysis conducted on amoA/AmoA sequences of reamplified TGGE bands showed, however, that closely related ammonia-oxidizing populations inhabited the sludge of the MBR in all experimental stages. Nitrosomonas cluster 7 populations ( N. europaea- N. eutropha cluster) prevailed under all conditions tested, even when the MBR was operated under complete biomass retention or at low temperatures, suggesting that the high ammonia concentrations in the system were determinant to select r-strategist AOB.
- Subjects
NITROSOMONAS; ULTRAFILTRATION; PHYSIOLOGICAL effects of ammonia; CLADISTIC analysis; METHODS in Electrophoresis
- Publication
Bioprocess & Biosystems Engineering, 2013, Vol 36, Issue 7, p901
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-012-0823-0