We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
金黄色葡萄球菌拮抗细菌的分离鉴定及拮抗活性分析.
- Authors
郑佩瑶; 田羽; 王娟; 邓丽; 肖丹; 刘建蕊; 韩雨霏; 李祝
- Abstract
In this study, 9 strains of bacteria with antagonistic activity against Staphylococcus aureus PJ-1 were obtained by isolation and screening from natural fermented mustard. The strains with good antagonistic activity were selected by point-joint method and Oxford cup method respectively, and the strains were identified by morphological observation and biology identification. The fermentation products of the screened strains were extracted with ethyl acetate for composition analysis by GC-MS. The results showed that three strains SC-3, SC-6, and SC-9 with better antagonistic effects on S. aureus PJ-1 were screened using two methods, and they were identified as Bacillus velezensis, Bacillus mojavensis, and Bacillus amyloliquefaciens, respectively. The crude extract of strain SC-3 from ethyl acetate had a good antagonistic effect on strain PJ-1, with an antibacterial rate of 75.04%. The composition of ethyl acetate extract of strain SC-3 was analyzed by GC-MS, it was found that the main substances were 10-hydroxy-2-decenoic acid (41.99%), 1, 4-diazazine, 5-dioxa-3-isobutyldicycloxylic acid [4.3.0] nonane (8.80%), 3-methyldioxy-piperazine (8.26%), L-leucine (5.83%), etc. It is suggested that the components of B. velezensis may be used in the control of foodborne S. aureus.
- Publication
China Brewing, 2023, Vol 42, Issue 11, p61
- ISSN
0254-5071
- Publication type
Article
- DOI
10.11882/j.issn.0254-5071.2023.11.010