We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
油茶果生刺盘孢组蛋白乙酰化转移酶 Rtt109 生物 学功能.
- Authors
王成玉; 姚权; 李河
- Abstract
Anthracnose is one of the most important diseases of Camellia oleifera, caused by Colletotrichum fructicola. As an important epigenetic modification, histone acetylation plays an important role in the regulation of a variety of biological processes, which is currently unclear in C. fructicola. The research aims at exploring the biological function of histone acetyltransferase Rtt109 in C. fructicola in order to elucidate the molecular mechanism of CfRtt109 and provide theoretical basis for the prevention and control of oil-tea tree anthracnose. Based on homologous recombination principle and the PEG-mediated transformation method, the mutant strain of ΔCfrtt109 and the complemented strains were obtained. It was found that the vegetative growth and conidiation of mutant was significantly lower than that of wild type and the complemented strain. Compared with the wild type and the complemented strain, ΔCfrtt109 was more sensitive to 5.0 mmol/L DTT and 0.01% MMS, additives to PDA medium. The expression levels of HAC1, SCJ1 and PDI1 were significantly upregulated in ΔCfrtt109. RNA-seq combined with RT-qPCR analysis showed that loss of CfRTT109 would alter the expression of genes involved in DNA damage repair. Furthermore, the pathogenicity of the ΔCfrtt109 was significantly reduced on the wounded oil-tea leaf and apple. To sum up, our results revealed the critical roles of Rtt109 in growth, conidiation, tolerance to ER stress and DNA damage stress and pathogenicity of C. fructicola.
- Subjects
HOMOLOGOUS recombination; HISTONE acetyltransferase; CAMELLIA oleifera; HISTONE acetylation; DNA repair; ANTHRACNOSE; HISTONES
- Publication
Mycosystema, 2024, Vol 43, Issue 4, p1
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.230275