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- Title
The Potential of Purple Sweet Potato-based Food Bar as an Immunomodulator and Hepatoprotector Against Diethylnitrosamine-induced Mice.
- Authors
Widyaningsih, Tri Dewanti; Sujuti, Hidayat; Fibrianto, Kiki; Honestin, Trifena; Sari Sigalingging, Catherine Novita
- Abstract
The food bar's effect as an immunomodulator and hepatoprotector in vivo was tested on male BALB/c mice induced with diethylnitrosamine (DEN). The experimental animals were treated using the Post-test Control Group Design method. Six groups of 30 mice each made up the sample: The negative control group of healthy mice fed with a normal diet (P0); the group of healthy mice given the food bar (P1); the positive control group of mice induced with DEN and given a normal diet (P2); the group of mice induced with DEN and given a normal diet + food bar at 11,700 mg/kg (P3); the group of mice induced with DEN and given a normal diet + food bar at 23,400 mg/kg (P4); and the group of mice induced with DEN given the food bar (P5). The observations conducted included the spleen, which was used for the analysis of immunomodulators on T CD4+CD25+, CD4+, and CD8+ cells with cytokines TNF-α, IFN-γ, and IL-10, blood serum for the analysis of SGPT and SGOT, and liver organ for the analysis of SOD, MDA, and histopathology. The results of the immunomodulator testing showed that the administration of the food bar (P5) significantly (p < 0.05) resulted in the highest percentage of CD4+ T cells at 2.93 % and CD8+ T cells at 5.30 %, a lower percentage of CD8+ T cells expressing proinflammatory cytokines (TNF-α at 0.27 % and IFN-γ at 0.29 %), a higher percentage of CD4+ T cells expressing CD25+, and CD25+ T cells expressing anti-inflammatory cytokines (IL-10) at 2.83 %. This group also exhibited the lowest levels of SGPT and SGOT, a higher percentage of SOD, and a lower percentage of MDA compared to other groups. The food bar, based on the mix of purple sweet potato, mung beans, moringa leaves, and strawberries, also reduced liver damage, as demonstrated through histopathological testing.
- Subjects
SWEET potatoes; SWEETNESS (Taste); BLOOD serum analysis; MUNG bean; T cells; MICE
- Publication
Trends in Sciences, 2024, Vol 21, Issue 5, p1
- ISSN
2774-0226
- Publication type
Article
- DOI
10.48048/tis.2024.7363