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- Title
荚膜甲基球菌蛋白遗传毒性研究和被动皮肤过敏试验.
- Authors
高嫣珺; 聂 雅; 陈秀云; 张思微; 刘 瑜; 卜仕金
- Abstract
Methylococcus capsulatus bacteria meal (MBM) is intended to be developed as a new feed ingredient. This study was aimed to evluate toxicological safety of MBM through genotoxicity study and passive cutaneous anaphylaxis test, which laying the foundation for its future applications. The Ames test was performed by the plate doping method with doses ranging from 0.625 to 10 mg per dish, reverse mutation test was carried out in five strains of TA97, TA98, TA100, TA102 and TA1535. The mice micronucleus test and the sperm malformation test both performed at 2500, 5000, 10000 mg/kg. The micronucleus test was conducted using a 30 h double administration method, and the sperm abnormality test was continuously exposed to the MBM for 5 days. Two tests were respectively sampled and produced smear at a predetermined time. The rat passive cutaneous anaphylaxis test was performed at 250, 500, 1000 mg/kg. The antiserum was prepared and then sensitization by Intradermal injection on the back. Antigenic challenge was performed 24 h and/or 48 h later. Finally the blue reaction spots were examined. Negative and positive control groups were set up for all tests. The results showed that the with or without metabolic activation system (S9), the Salmonella typhimurium reverse mutation assay (Ames test) result was negative; the mice bone marrow cell micronucleus and sperm abnormality test results were both negative; and the rat passive cutaneous anaphylaxis test result was negative. The study suggests that MBM showed no genotoxicity and potential allergy under the set test system and dose.
- Subjects
BONE marrow cells; AMES test; SKIN tests; BIOTRANSFORMATION (Metabolism); INTRADERMAL injections
- Publication
Feed Industry, 2023, Vol 44, Issue 21, p88
- ISSN
1001-991X
- Publication type
Article
- DOI
10.13302/j.cnki.fi.2023.21.014